AVIDIN-BIOTIN BASED ELECTROCHEMICAL IMMUNOASSAY FOR THYROTROPIN

被引：43

作者：

ATHEY, D

BALL, M

MCNEIL, CJ

机构：

[1] Department of Clinical Biochemistry, The Medical School, Framlington Place, University of Newcastle Upon Tyne

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1993年 / 30卷

关键词：

1-NAPHTHYL PHOSPHATE; ALKALINE PHOSPHATASE; AMPEROMETRY; DISPOSABLE SCREEN-PRINTED ELECTRODES;

D O I：

10.1177/000456329303000608

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.

引用

页码：570 / 577

页数：8

共 15 条

[1]

ATHEY D, 1993, THESIS U NEWCASTLE T

[2] STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].