DETECTION OF GIANT MYOFIBRILLAR PROTEINS CONNECTIN AND NEBULIN BY ELECTROPHORESIS IN 2-PERCENT POLYACRYLAMIDE SLAB GELS STRENGTHENED WITH AGAROSE

被引：96

作者：

TATSUMI, R

HATTORI, A

机构：

[1] Hokkaido Univ, Fac Agr, Dept Anim Sci, Sapporo, Hokkaido, 060, Kita Ku

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 224卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution. (C) 1995 Academic Press, Inc.

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页码：28 / 31

页数：4

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