MOLECULAR ANALYSIS OF THE CIP1/WAF1 (P21) GENE IN DIVERSE TYPES OF HUMAN TUMORS

被引：45

作者：

WATANABE, H ^{[1
]}

FUKUCHI, K ^{[1
]}

TAKAGI, Y ^{[1
]}

TOMOYASU, S ^{[1
]}

TSURUOKA, N ^{[1
]}

GOMI, K ^{[1
]}

机构：

[1] SHOWA UNIV, SCH MED, DEPT HEMATOL, TOKYO 142, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1263卷 / 03期

关键词：

CIP1/WAF1 (P21) GENE; CYCLIN-DEPENDENT KINASE INHIBITOR; TUMOR SUPPRESSOR GENE; PCR-SSCP;

D O I：

10.1016/0167-4781(95)00110-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have screened for mutations in the Cip1/Waf1 gene using Southern blot analysis and the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method in diverse human tumors. Seven of 102 (7%) human tumor samples were identified to have point mutations within the coding region of the Cip1/Waf1 gene. Two of the seven mutated cases showed gene rearrangements. These results suggest that the frequency of genetic alterations in the Cip1/Waf1 gene is relatively low in comparison with several known tumor suppressor genes.

引用

页码：275 / 280

页数：6

共 21 条

[1]

CHEDID M, 1994, ONCOGENE, V9, P3021

[2] WAF1, A POTENTIAL MEDIATOR OF P53 TUMOR SUPPRESSION [J].