DEVELOPMENT OF GUSA REPORTER GENE CONSTRUCTS FOR CEREAL TRANSFORMATION - AVAILABILITY OF PLANT TRANSFORMATION VECTORS FROM THE CAMBIA MOLECULAR-GENETIC RESOURCE SERVICE

被引：41

作者：

MCELROY, D

CHAMBERLAIN, DA

MOON, E

WILSON, KJ

机构：

[1] CTR APPLICAT MOLEC BIOL INT AGR, CANBERRA, ACT 2601, AUSTRALIA

[2] CSIRO, DIV PLANT IND, CANBERRA, ACT 2601, AUSTRALIA

[3] AJOU UNIV, DEPT BIOL, SUWON, SOUTH KOREA

来源：

MOLECULAR BREEDING | 1995年 / 1卷 / 01期

关键词：

REPORTER GENES; EXPRESSION VECTORS; CEREAL TRANSFORMATION; BETA-GLUCURONIDASE; WHEAT; BARLEY;

D O I：

10.1007/BF01682087

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The use of reporter genes to characterise sequence elements that act to regulate gene expression in transgenic plants has been vital to the development of foreign gene expression strategies for use in cereal transformation. The gusA locus of Escherichia coli, which encodes the enzyme beta-glucuronidase (GUS), is by far the most popular reporter gene used in plant transformation. In this paper we extend the utility of the GUS reporter gene system in cereal transformation by describing and evaluating a number of novel constructs suitable for use in direct gene transfer experiments. These plasmids are all available from the Molecular Genetic Resource Service of the Center for the Application of Molecular Biology to International Agriculture.

引用

页码：27 / 37

页数：11

共 36 条

[1] TRANSGENIC SUGARCANE PLANTS VIA MICROPROJECTILE BOMBARDMENT [J].