A SIMPLE, RAPID STAINING PROCEDURE FOR METHACRYLATE EMBEDDED TISSUE-SECTIONS USING CHROMOTROPE-2R AND METHYLENE-BLUE

被引：13

作者：

DOUGHERTY, MM ^{[1
]}

KING, JS ^{[1
]}

机构：

[1] MED UNIV S CAROLINA, DEPT ANAT, CHARLESTON, SC 29425 USA

来源：

STAIN TECHNOLOGY | 1984年 / 59卷 / 03期

关键词：

D O I：

10.3109/10520298409113848

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sections of tissue embedded in glycol methacrylate can be stained in rapid sequence with solutions of 1% aqueous chromotrope 2R adjusted to pH 3 and 0.1% methylene blue to produce sufficient contrast and cellular detail to permit quick visual inspection and/or photomicrography. Solutions of these strains are simple to prepare and are stable over long periods. Staining of sections may be accomplished within 6 min.

引用

页码：149 / 153

页数：5