STRONGYLOIDES-STERCORALIS - ANTIGENIC ANALYSIS OF INFECTIVE LARVAE AND ADULT WORMS

被引：18

作者：

NORTHERN, C ^{[1
]}

GROVE, DI ^{[1
]}

机构：

[1] SIR CHARLES GAIRDNER HOSP,DEPT POSTGRAD MED EDUC,NEDLANDS,WA 6009,AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1990年 / 20卷 / 03期

基金：

英国医学研究理事会;

关键词：

adult worms; antigens; human sera; infective larvae; stage-specificity; Strongyloides stercoralis; Western blots;

D O I：

10.1016/0020-7519(90)90155-G

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The protein composition of Strongyloides stercoralis infective larvae and adult worms solubilized sequentially in water, sodium deoxycholate and sodium dodecyl sulphate (SDS) and their excretory/secretory products were analysed by one- and two-dimensional SDS-polyacrylamide electrophoresis. These extracts were demonstrated to be complex mixtures containing many proteins, some of which were common and others which were stage-specific. Western blot analysis of these antigens with infected human sera showed most sero-reactivity against larval antigens, whilst normal human sera were unreactive. These data identify immunogenic antigens which may be available for detection in an antigen assay. © 1990.

引用

页码：381 / 387

页数：7

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