PROGESTERONE RECEPTORS IN RAT-BRAIN AND UTERUS - DEPENDENCE ON THE HORMONAL MILIEU

被引:7
作者
ALKHOURI, H
GREENSTEIN, BD
机构
关键词
D O I
10.1677/joe.0.1070159
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cytosols were incubated from the hypothalamus and mid-brain and from the uterus, and incubated with [3H]progesterone alone or in the presence of excess radioinert steroid to reveal saturable binding sites. Bound and free hormone were separated by gel filtration. Scatchard analysis of the binding sites yielded evidence for only one class of binding sites of high affinity and limited capacity. The binding components in the hypothalamus and uterus appeared to fluctuate during the oestrous cycle, attaining a nadir at metoestrus, while those in the mid-brain were apparently unchanged. During pregnancy hypothalamic [3H]progesterone-binding sites appeared to loss affinity for the steroid while in the uterus the affinity for the steroid was unchanged but the absolute numbers of binding sites were greatly increased at day 10. It is concluded, both from studies of the properties intrinsic to the binding reaction and from endocrine correlates, that the macromolecular progesterone-binding components in the brain may be receptors for the hormone and that there may be differences between the properties of progesterone receptors in different tissues. Furthermore, during pregnancy there may be qualitative changes in the neural progesterone receptors which are not mediated by oestradiol.
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页码:159 / 162
页数:4
相关论文
共 17 条
[1]   ROLE OF CORTICOSTEROID-BINDING GLOBULIN IN INTERACTION OF CORTICOSTERONE WITH UTERINE AND BRAIN PROGESTERONE RECEPTORS [J].
ALKHOURI, H ;
GREENSTEIN, BD .
NATURE, 1980, 287 (5777) :58-60
[2]  
ALKHOURI H, 1982, J PHYSIOL-LONDON, V332, pP110
[3]   INHIBITORY EFFECTS OF VARIOUS PROGESTINS AND DEOXYCORTICOSTERONE ON RAPID ONSET OF MATERNAL-BEHAVIOR INDUCED BY OVARIECTOMY-HYSTERECTOMY DURING LATE PREGNANCY IN RATS [J].
BRIDGES, RS ;
FEDER, HH .
HORMONES AND BEHAVIOR, 1978, 10 (01) :30-39
[4]   MODULATION OF PROGESTERONE CONCENTRATION IN MYOMETRIUM OF PREGNANT RAT BY CHANGES IN CYTOPLASMIC RECEPTOR PROTEIN ACTIVITY [J].
DAVIES, IJ ;
RYAN, KJ .
ENDOCRINOLOGY, 1973, 92 (02) :394-401
[5]   UPTAKE OF PROGESTERONE BY UTERUS OF PREGNANT RAT IN-VIVO AND ITS RELATIONSHIP TO CYTOPLASMIC PROGESTERONE-BINDING PROTEIN [J].
DAVIES, IJ ;
RYAN, KJ .
ENDOCRINOLOGY, 1972, 90 (02) :507-+
[6]   CURRENT MODELS OF STEROID-HORMONE ACTION - CRITIQUE [J].
GORSKI, J ;
GANNON, F .
ANNUAL REVIEW OF PHYSIOLOGY, 1976, 38 :425-450
[7]   EVIDENCE FOR SPECIFIC PROGESTERONE RECEPTORS IN RAT-BRAIN CYTOSOL [J].
GREENSTEIN, BD .
JOURNAL OF ENDOCRINOLOGY, 1978, 79 (03) :327-338
[8]   MECHANISM OF ACTION OF FEMALE SEX-HORMONES [J].
JENSEN, EV ;
DESOMBRE, ER .
ANNUAL REVIEW OF BIOCHEMISTRY, 1972, 41 :203-+
[9]   SPECIFIC PROGESTERONE RECEPTORS IN HYPOTHALAMUS AND ANTERIOR HYPOPHYSIS OF RAT [J].
KATO, J ;
ONOUCHI, T .
ENDOCRINOLOGY, 1977, 101 (03) :920-928
[10]  
King R.J., 1974, Steroid - Cell Interactions