THE MOUSE 5-HT1C RECEPTOR CONTAINS 8 HYDROPHOBIC DOMAINS AND IS X-LINKED

被引：64

作者：

YU, L

NGUYEN, H

LE, H

BLOEM, LJ

KOZAK, CA

HOFFMAN, BJ

SNUTCH, TP

LESTER, HA

DAVIDSON, N

LUBBERT, H

机构：

[1] UNIV BRITISH COLUMBIA,BIOTECHNOL LAB,VANCOUVER V6T 1W5,BC,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT NEUROSCI,VANCOUVER V6T 1W5,BC,CANADA

[3] UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER V6T 1W5,BC,CANADA

[4] CALTECH,DIV BIOL,PASADENA,CA 91125

[5] CALTECH,DIV CHEM & CHEM ENGN,PASADENA,CA 91125

[6] INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,INDIANAPOLIS,IN 46202

[7] NIID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892

[8] SANDOZ LTD,PRECLIN RES,CH-4002 BASEL,SWITZERLAND

[9] NIMH,CELL BIOL LAB,BETHESDA,MD 20892

来源：

MOLECULAR BRAIN RESEARCH | 1991年 / 11卷 / 02期

关键词：

G-PROTEIN-COUPLED RECEPTOR; X-CHROMOSOME; XENOPUS OOCYTE;

D O I：

10.1016/0169-328X(91)90116-F

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) exerts diverse physiological effects in the central and peripheral nervous systems and in smooth muscle by interacting with pharmacologically distinct membrane receptors. We report here the cDNA cloning of the mouse 5-HT1c receptor and its functional expression in Xenopus oocytes. This receptor possesses the unusual feature of containing eight hydrophobic domains capable of forming membrane-spanning a-helices, contrary to the usual '7-helix' paradigm for other membrane receptors that function through coupling to GTP-binding proteins. By hybridization analysis of Chinese hamster x mouse somatic cell hybrid fines, the gene for the receptor, designated Htr1c, has been assigned to the mouse X chromosome.

引用

页码：143 / 149

页数：7

共 48 条

[1] STRUCTURAL REQUIREMENTS OF N-GLYCOSYLATION OF PROTEINS - STUDIES WITH PROLINE PEPTIDES AS CONFORMATIONAL PROBES [J].