INVITRO EVALUATION OF 5-LIPOXYGENASE AND CYCLOOXYGENASE INHIBITORS USING BOVINE NEUTROPHILS AND PLATELETS AND HPLC

被引：48

作者：

DANNHARDT, G

LEHR, M

机构：

[1] Institut für Pharmazeutische Chemie der Johann Wolfgang Goethe-Universitäl, Frankfurt, 6000

来源：

JOURNAL OF PHARMACY AND PHARMACOLOGY | 1992年 / 44卷 / 05期

关键词：

D O I：

10.1111/j.2042-7158.1992.tb03636.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Inhibition of 5-lipoxygenase has been determined by monitoring the formation of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in bovine polymorphonuclear leucocytes. For evaluating the inhibition of cyclo-oxygenase two different test systems are presented: the first uses 12-hydroxyheptadecatrienoic acid produced by bovine platelets as an indicator of the cyclo-oxygenase activity; the second test system monitors the prostaglandin E, formation by bovine platelets. All arachidonic acid metabolites are quantified by reverse-phase HPLC with UV-detection.

引用

页码：419 / 424

页数：6

共 38 条

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BAILEY JM, 1985, PROSTAGLANDINS LEUKO

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