ACTION PATTERN OF STREPTOMYCES-GRISEUS CHITINASE ON PARTIALLY N-ACETYLATED CHITOSAN

被引：41

作者：

OHTAKARA, A

MATSUNAGA, H

MITSUTOMI, M

机构：

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 12期

关键词：

D O I：

10.1080/00021369.1990.10870469

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main oligosaccharides were identified by N-acetylation, exo-splitting with beta-GlcNAcase and beta-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)2, and (GlcNAc)3, hetero-chitooligosaccharides such as GlcN . GlcNAc, GlcN . GlcNAc . GlcNAc, GlcN . GlcN. GlcNAc, GlcN . GlcNAc . GlcNAc . GlcNAc, GlcNAc . GlcN . GlcNAc . GlcNAc, GlcN . GlcNAc . GlcN . GlcNAc, and GlcN . GlcN . GlcNAc . GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the N-acetyl-beta-D-glucosaminidic linkages in partially N-acetylated chitosan.

引用

页码：3191 / 3199

页数：9

共 17 条

[1] ACTION OF LYSOZYME ON PARTIALLY DEACETYLATED CHITIN
AMANO, KI
ITO, E
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 85 (01): : 97 - 104
[2] CHITIN OLIGOSACCHARIDES ELICIT LIGNIFICATION IN WOUNDED WHEAT LEAVES
BARBER, MS
BERTRAM, RE
RIDE, JP
[J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1989, 34 (01) : 3 - 12
[3] AMINO-SUGARS AND RELATED COMPOUNDS .4. ISOLATION AND PROPERTIES OF OLIGOSACCHARIDES OBTAINED BY CONTROLLED FRAGMENTATION OF CHITIN
BARKER, SA
FOSTER, AB
STACEY, M
WEBBER, JM
[J]. JOURNAL OF THE CHEMICAL SOCIETY, 1958, (JUN): : 2218 - 2227
[4] FENTON DM, 1981, J GEN MICROBIOL, V126, P151
[5] EFFECTS OF THE N-SUBSTITUTION AND PHYSICAL FORM OF CHITOSAN AND THE MODIFICATION OF ITS NON-REDUCING END GROUPS ON THE RATES OF HYDROLYSIS BY CHITINASE FROM STREPTOMYCES-GRISEUS
HIRANO, S
YAGI, Y
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (04): : 963 - 964
[6] SIMPLE ACTIVITY MEASUREMENT OF LYSOZYME
IMOTO, T
YAGISHITA, K
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1971, 35 (07): : 1154 - +
[7] CHARACTERIZATION OF THE SMALLEST CHITOSAN OLIGOMER THAT IS MAXIMALLY ANTIFUNGAL TO FUSARIUM-SOLANI AND ELICITS PISATIN FORMATION IN PISUM-SATIVUM
KENDRA, DF
HADWIGER, LA
[J]. EXPERIMENTAL MYCOLOGY, 1984, 8 (03): : 276 - 281
[8] KOBAYASHI T, 1988, NIPPON NOGEIK KAISHI, V62, P1471
[9] KOBAYASHI T, 1988, NIPPON NOGEIK KAISHI, V62, P1463
[10] KURITA K, 1977, MAKROMOL CHEM, V178, P3197

← 1 2 →