THE LIQUID-LIQUID MICRO-INTERFACE FOR THE AMPEROMETRIC DETECTION OF UREA

被引：38

作者：

OSBORNE, MD

GIRAULT, HH

机构：

[1] Laboratoire d'Electrochimie, Ecole Polytechnique Fédérale de Lausanne, Lausanne

来源：

ELECTROANALYSIS | 1995年 / 7卷 / 08期

关键词：

UREA; LIQUID-LIQUID INTERFACE; ENZYMATIC ASSAY;

D O I：

10.1002/elan.1140070804

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The detection of the ammonium ion produced by the enzymatic action of urease on urea is investigated electrochemically by means of facilitated ion transfer reactions at liquid-liquid micro-interfaces. The urease was used either in a dissolved form (free in aqueous solution) or immobilized onto the surface of an ammonia gas permeable membrane. The sensing structure utilizing the ammonia gas permeable membrane allowed the detection of urea from 200 mu M to 4mM. Thick film technology was utilized in conjunction with UV excimer laser photoablation techniques to fabricate a microelectrode strip sensor for the ammonium ion.

引用

页码：714 / 721

页数：8

共 23 条

[1] MASS-PRODUCTION OF BIOSENSORS [J].