EXTRACTION AND MEASUREMENT OF ADENOSINE-TRIPHOSPHATE FROM MARINE-SEDIMENTS

被引：59

作者：

BANCROFT, K ^{[1
]}

PAUL, EA ^{[1
]}

WIEBE, WJ ^{[1
]}

机构：

[1] UNIV GEORGIA, DEPT MICROBIOL, ATHENS, GA 30602 USA

来源：

LIMNOLOGY AND OCEANOGRAPHY | 1976年 / 21卷 / 03期

关键词：

D O I：

10.4319/lo.1976.21.3.0473

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A technique was developed, using boiling NaHCO3 buffer, to extract ATP from marine sediments and has been tested on a variety of sediments, including those with high organic content, clay and carbonate. Recovery of ATP, as measured by the addition of bacteria of known ATP content to sediment, varied from 64-100%. The technique also was as effective as the conventional Tris buffer for extraction of ATP from both pure cultures of bacteria grown in broth and natural seawater samples.

引用

页码：473 / 479

页数：7

共 17 条

[1]

AUSMUS BS, 1973, B ECOL RESEARCH COMM, V17, P223

[2] ADENYLATE ENERGY CHARGE IN ESCHERICHIA-COLI DURING GROWTH AND STARVATION [J].