NUCLEAR LIPIDS IN FRIEND-CELLS - SHIFTED PROFILE OF DIACYLGLYCEROL DURING ERYTHROID-DIFFERENTIATION INDUCED BY DMSO

被引：19

作者：

TRUBIANI, O

MARTELLI, AM

MANZOLI, L

SANTAVENERE, E

COCCO, L

机构：

[1] UNIV CHIETI, CNR, INST CYTOMORPHOL, CHIETI, ITALY

[2] UNIV CHIETI, INST HUMAN MORPHOL, CHIETI, ITALY

[3] UNIV BOLOGNA, INST HUMAN ANAT, I-40126 BOLOGNA, ITALY

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1990年 / 14卷 / 06期

关键词：

D O I：

10.1016/0309-1651(90)91183-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Friend cells were labelled for 90 min. with [3H]-Glycerol and the radioactivity in DAG# # Abbrevations used: DMSO, dimethylsulphoxide; DAG, diacylglycerol; TG, triacylglycerol; TLC, thin layer chromatography. and TG was measured. The relative percentage of radiolabelled DAG differs in isolated nuclei as compared to intact cells. Moreover the level of newly synthesized DAG decreases in nuclei isolated from cells treated with DMSO for 24 hrs. as well as in nuclei from cells treated for 96 hrs. and terminally differentiated in the erythroid pathway. Since these changes are not seen in intact cells, the results are consistent with the hypothesis that nuclear lipids and namely the products of polyphosphoinositide hydrolysis, such as DAG, are involved in the regulation of the processes leading to cell differentiation. © 1990.

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页码：559 / 566

页数：8

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