SYNTHESIS AND ANTIBODY-MEDIATED DETECTION OF OLIGONUCLEOTIDES CONTAINING MULTIPLE 2,4-DINITROPHENYL REPORTER GROUPS

被引：12

作者：

GRZYBOWSKI, J

WILL, DW

RANDALL, RE

SMITH, CA

BROWN, T

机构：

[1] UNIV EDINBURGH, EDINBURGH CTR MOLEC RECOGNIT, DEPT CHEM, W MAINS RD, EDINBURGH EH9 3JJ, MIDLOTHIAN, SCOTLAND

[2] UNIV ST ANDREWS, DEPT BIOCHEM & MICROBIOL, ST ANDREWS KY16 9AL, SCOTLAND

[3] CRUACHEM LTD, GLASGOW G20 0UA, SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 08期

关键词：

D O I：

10.1093/nar/21.8.1705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of non-nucleoside-based 2,4-dinitrophenyl (DNP) phosphoramidites have been prepared and used in the multiple labelling of oligonucleotides during solid-phase synthesis. The length of spacer arm between the DNP label and the oligonucleotide phosphate backbone, and the number of attached DNP groups have both been varied in order to determine the optimum conditions for anti-DNP antibody binding. Detection using enzyme-linked colorimetric techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.

引用

页码：1705 / 1712

页数：8

共 27 条

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