ISOLATED TUMOR-GROWTH IN A SURGICALLY FORMED SKIN PEDICLE IN THE RAT - A NEW TUMOR-MODEL FOR NMR-STUDIES

被引：43

作者：

HAHN, EW ^{[1
]}

PESCHKE, P ^{[1
]}

MASON, RP ^{[1
]}

BABCOCK, EE ^{[1
]}

ANTICH, PP ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT RADIOL,5323 HARRY HINES BLVD,DALLAS,TX 75235

来源：

MAGNETIC RESONANCE IMAGING | 1993年 / 11卷 / 07期

关键词：

SKIN PEDICLE; DUNNING PROSTATE TUMOR; NMR; MRI;

D O I：

10.1016/0730-725X(93)90219-4

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

We have developed a new tumor model, the skin flap pedicle, which is ideally suited for study by such techniques as magnetic resonance imaging (MRI) and spectroscopy (MRS) and positron emission tomography (PET) to evaluate changes in metabolism/physiology in tumors before and after treatment. Heretofore, results from such studies were generally obtained from tumors growing in the thigh or flank region and these sites of growth often compromised the quality of the results, because of the contribution of normal tissues such as muscle and bone. In an attempt to circumvent such problems, we developed a surgical technique for creating a tubular skin pedicle and subsequently transplanting tumors in the lumen of such pedicles. We have successfully grown Dunning Prostate tumors (R-3327) as PEDICLE-TUMORS (PED-TUM). This paper describes the surgical procedure for formation of the skin pedicle, implantation of the tumor, data on the growth and histology and discusses the application of the PED-TUM to research problems. We compare the NMR characteristics of the tumor grown in the traditional SC thigh position with that grown in the pedicle and demonstrate the utility and advantages of the new model for studies of tumor physiological function and structure.

引用

页码：1007 / 1017

页数：11

共 26 条

[1]

Isaacs, Heston, Weissman, Coffey, Animal models of the hormone-sensitive and -insensitive prostatic adenocarcinomas, Dunning R3327-H, R3327-HI and R-3327-AT, Cancer Res., 38, pp. 4353-4359, (1978)

[2]

Hahn, Wolber, Bak, Hoever, Gerlach, Volm, Lorenz, Response of a Dunning prostate tumor to fast neutrons, Strahlenther, 165, pp. 283-285, (1989)

[3]

Corbett, Nunnally, Giovanella, Antich, Characterization of the31P nmr spectra from human melanoma tumors implanted in nude mice, Cancer Res., 47, pp. 5065-5069, (1987)

[4]

Evanochko, Ng, Glickson, Application of in vivo NMR spectroscopy to cancer, Magn. Reson. Med., 1, pp. 508-534, (1984)

[5]

Griffiths, Bhujwalla, Coombes, Maxwell, Midwood, Morgan, Nias, Perry, Prior, Prysor-Jones, Rodrigues, Stubbs, Tozer, Monitoring cancer therapy by NMR spectroscopy, Ann. NY Acad. Sci., 508, pp. 182-199, (1988)

[6]

Antich, Mason, Nunnally, Applications of magnetic resonance techniques to deep tumor hyperthermia, Strahlenther. Onkol., 165, pp. 734-737, (1989)

[7]

Sostman, Rockwell, Smith, Gore, Kennedy, Habib, Fischer, Armitage, Holcomb, Magnetic resonance, pathology and physiology of BA 1112 rhabdomyosarcoma in vivo, Invest. Radiol., 23, pp. 277-280, (1988)

[8]

Vaupel, Okunieff, Neuringer, In vivo 31P-NMR spectroscopy of murine tumours before and after localized hyperthermia, Int. J. Hyperthermia, 6, pp. 15-31, (1990)

[9]

Fu, Wendland, Iyer, Lam, Engeseth, James, Correlations between in vivo 31P NMR spectroscopy measurements, tumor size, hypoxic fraction and cell survival after radiotherapy, Int. J. Radiat. Oncol. Biol. Phys., 18, pp. 1341-1350, (1990)

[10]

Sijens, Bovee, Seijkens, Koole, Los, van Rijssel, Murine mammary tumor response to hyperthermia and radiotherapy evaluated by in vivo 31P-nuclear magnetic-resonance spectroscopy, Cancer Res., 47, pp. 6467-6473, (1987)

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