A RAPID ASSAY FOR 17-ALPHA-OH-PROGESTERONE IN PLASMA, SALIVA AND AMNIOTIC-FLUID USING A MAGNETIZABLE SOLID-PHASE ANTISERUM

被引:31
作者
DYAS, J
READ, GF
GUHAMAULIK, T
HUGHES, IA
RIADFAHMY, D
机构
[1] UNIV WALES WELSH NATL SCH MED, TENOVUS INST CANC RES, CARDIFF CF4 4XX, S GLAM, WALES
[2] UNIV WALES WELSH NATL SCH MED, DEPT CHILD HLTH, CARDIFF CF4 4XX, S GLAM, WALES
关键词
D O I
10.1177/000456328402100514
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A radioimmunoassay suitable for measurement of 17.alpha.OH-progesterone concentrations in small aliquots of plasma (20 .mu.l), amniotic fluid (20 .mu.l) and saliva (200 .mu.l) is described. The assay features an antiserum raised against a 17.alpha.OH-progesterone-3-(O-carboxymethyl)oxime/BSA [bovine serum albumin] conjugate coupled to a magnetizable, solid-phase support; the homologous radioligand is a 125I-iodohistamine conjugate. This combination of a .gamma.-emitting ligand and a magnetic-separation procedure has the advantage of reducing assay time and cost; it also allows processing of plasma and saliva samples in the same assay batch. The method has satisfactory sensitivity, precision and accuracy. Data derived from clinical studies of patients and congenital adrenal hyperplasia indicate the usefulness of this assay in routine practice.
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页码:417 / 424
页数:8
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