EFFECTS OF DIFFERENT ENZYMATIC TREATMENTS ON THE RELEASE OF TITIN FRAGMENTS FROM RABBIT SKELETAL MYOFIBRILS - PURIFICATION OF AN 800 KDA TITIN POLYPEPTIDE

被引：23

作者：

ASTIER, C ^{[1
]}

LABBE, JP ^{[1
]}

ROUSTAN, C ^{[1
]}

BENYAMIN, Y ^{[1
]}

机构：

[1] UNIV MONTPELLIER 1,EPHE,MOTIL CELLULAIRE LAB,CTR RECH BIOCHIM MACROMOLEC,CNRS,LP 9008,F-34060 MONTPELLIER,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1993年 / 290卷

关键词：

D O I：

10.1042/bj2900731

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In myofibrils, titin (also called connectin) molecules span from Z line to M line and constitute a third filament system containing an elastic domain in the I band. This giant protein is particularly sensitive to proteolysis in situ. Treatment of rabbit skeletal myofibrils with exogenous proteinases induces a release of titin fragments, which are detected in the soluble myofibrillar fraction. The cleavage of titin occurs at specific points localized at the proximity of Z Iine and could lead to a concomitant release of alpha-actinin.

引用

页码：731 / 734

页数：4

共 30 条

[1] ASTIER C, 1991, COMP BIOCH PHYSL B, V100, P465
[2] THE ORGANIZATION OF TITIN FILAMENTS IN THE HALF-SARCOMERE REVEALED BY MONOCLONAL-ANTIBODIES IN IMMUNOELECTRON MICROSCOPY - A MAP OF 10 NONREPETITIVE EPITOPES STARTING AT THE Z-LINE EXTENDS CLOSE TO THE M-LINE
FURST, DO
OSBORN, M
NAVE, R
WEBER, K
[J]. JOURNAL OF CELL BIOLOGY, 1988, 106 (05) : 1563 - 1572
[3] GOLL DE, 1991, J BIOL CHEM, V266, P8501
[4] CHANGES IN CONTRACTILE PROPERTIES WITH SELECTIVE DIGESTION OF CONNECTIN (TITIN) IN SKINNED FIBERS OF FROG SKELETAL-MUSCLE
HIGUCHI, H
[J]. JOURNAL OF BIOCHEMISTRY, 1992, 111 (03) : 291 - 295
[5] HIGUCHI H, 1992, J BIOCHEM-TOKYO, V111, pA426
[6] THE POSITIONAL STABILITY OF THICK FILAMENTS IN ACTIVATED SKELETAL-MUSCLE DEPENDS ON SARCOMERE-LENGTH - EVIDENCE FOR THE ROLE OF TITIN FILAMENTS
HOROWITS, R
PODOLSKY, RJ
[J]. JOURNAL OF CELL BIOLOGY, 1987, 105 (05) : 2217 - 2223
[7] A PHYSIOLOGICAL-ROLE FOR TITIN AND NEBULIN IN SKELETAL-MUSCLE
HOROWITS, R
KEMPNER, ES
BISHER, ME
PODOLSKY, RJ
[J]. NATURE, 1986, 323 (6084) : 160 - 164
[8] ELASTIC BEHAVIOR OF CONNECTIN FILAMENTS DURING THICK FILAMENT MOVEMENT IN ACTIVATED SKELETAL-MUSCLE
HOROWITS, R
MARUYAMA, K
PODOLSKY, RJ
[J]. JOURNAL OF CELL BIOLOGY, 1989, 109 (05) : 2169 - 2176
[9] EXTENSIBLE AND LESS-EXTENSIBLE DOMAINS OF CONNECTIN FILAMENTS IN STRETCHED VERTEBRATE SKELETAL-MUSCLE SARCOMERES AS DETECTED BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY USING MONOCLONAL-ANTIBODIES
ITOH, Y
SUZUKI, T
KIMURA, S
OHASHI, K
HIGUCHI, H
SAWADA, H
SHIMIZU, T
SHIBATA, M
MARUYAMA, K
[J]. JOURNAL OF BIOCHEMISTRY, 1988, 104 (04) : 504 - 508
[10] ROLE OF CA++-DEPENDENT PROTEASES AND LYSOSOMAL-ENZYMES IN POSTMORTEM CHANGES IN BOVINE SKELETAL-MUSCLE
KOOHMARAIE, M
BABIKER, AS
MERKEL, RA
DUTSON, TR
[J]. JOURNAL OF FOOD SCIENCE, 1988, 53 (05) : 1253 - 1257

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