CYTOCHROME-C-OXIDASE IN ALZHEIMERS-DISEASE BRAIN - PURIFICATION AND CHARACTERIZATION

被引：138

作者：

PARKER, WD ^{[1
]}

PARKS, JK ^{[1
]}

机构：

[1] UNIV VIRGINIA,SCH MED,DEPT PEDIAT,CHARLOTTESVILLE,VA 22908

来源：

NEUROLOGY | 1995年 / 45卷 / 03期

关键词：

D O I：

10.1212/WNL.45.3.482

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Cytochrome c oxidase (COX) is deficient in both peripheral tissue and brain of Alzheimer's disease (AD) patients and may be of pathogenic significance in AD. We purified COX from AD brains (n = 3) and control brains (n = 3) and characterized the enzyme kinetically and spectrally. Purified AD brain COX displayed anomalous kinetic behavior compared with control brain COX in that the low K-m binding site was kinetically unidentifiable. For purposes of comparison, we purified COX from a standard beef heart preparation and found normal kinetic behavior. AD brain COX may be structurally abnormal and may make an important contribution to the bioenergetic defect seen in AD.

引用

页码：482 / 486

页数：5

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