INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN-1 INHIBITS THE DNA AMPLIFICATION INDUCED BY INSULIN-LIKE GROWTH FACTOR-I IN HUMAN GRANULOSA LUTEAL CELLS

被引：71

作者：

ANGERVO, M

KOISTINEN, R

SUIKKARI, AM

SEPPALA, M

机构：

[1] Departments I and II of Obstetrics and Gynecology, Helsinki University Central Hospital

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 06期

基金：

芬兰科学院;

关键词：

GRANULOSA CELL CULTURE; HUMAN; INSULIN-LIKE GROWTH FACTOR; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-1;

D O I：

10.1093/oxfordjournals.humrep.a137426

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

In order to study the effects of insulin-like growth factor (IGF-I) and insulin-like growth factor binding protein (IGFBP-1) on human granulosa cell proliferation after in vitro fertilization, cells were obtained after oocyte retrieval and cultured in the presence or absence of graded amounts of recombinant IGF-I, purified IGFBP-1 and [H-3]thymidine. Physiological concentrations of IGF-I (2-200 ng/ml) were found to stimulate [H-3]thymidine incorporation into the cells in a concentration-dependent manner. Half-maximal stimulation of [H-3]thymidine incorporation was obtained with 10 ng/ml exogenous IGF-I, which was chosen for suppression experiments with graded amounts of purified IGFBP-1. Suppression of IGF-stimulated thymidine incorporation was observed when 200 ng/ml or more of IGFBP-1 was added to the culture medium. The same concentration of IGFBP-1 also markedly inhibited binding of [I-125]iodotyrosyl IGF-I to the cells. It is concluded that: (i) after a refractory period, granulosa cells from hyperstimulated follicles retained their mitogenic activity; (ii) IGF-I is capable of stimulating DNA amplification in granulosa cells; and (iii) IGFBP-1 inhibits the IGF-I stimulated proliferation in these cells. In view of our previous studies showing that IGFBP-1 is synthesized by the granulosa cells as they luteinize, the present results suggest that IGFBP-1 is one of the endogenous factors locally regulating the growth and differentiation of granulosa cells.

引用

页码：770 / 773

页数：4

共 16 条

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