GEMINIVIRUS-BASED SHUTTLE VECTORS CAPABLE OF REPLICATION IN ESCHERICHIA-COLI AND MONOCOTYLEDONOUS PLANT-CELLS

被引：11

作者：

KAMMANN, M ^{[1
]}

MATZEIT, V ^{[1
]}

SCHMIDT, B ^{[1
]}

SCHELL, J ^{[1
]}

WALDEN, R ^{[1
]}

GRONENBORN, B ^{[1
]}

机构：

[1] CNRS,INST SCI VEGETALES,F-91198 GIF SUR YVETTE,FRANCE

来源：

GENE | 1991年 / 104卷 / 02期

关键词：

GENETIC ENGINEERING; SUSPENSION CULTURE CELLS; TRITICUM-MONOCOCCUM PROTOPLASTS; PLANT VIRUS; TRANSFECTION; RECOMBINANT DNA; PROKARYOTIC AND EUKARYOTIC VECTORS; TRANSPOSON TRAP;

D O I：

10.1016/0378-1119(91)90257-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Shuttle vectors have been constructed that are able to replicate in either Escherichia coli or plant cells. They contain the ColE1 origin of replication and parts of the wheat dwarf virus genome, a geminivirus infecting a variety of species of monocotyledonous plants. Such plasmids are able to replicate in E. coli and wheat cells. The plasmids can be rescued in E. coli and show no changes during their passage through plant cells. Such an E. coli/plant cell shuttle vector system could be used for the amplification of foreign genes in plant cells, for studies on DNA rearrangement or the isolation of plant transposons.

引用

页码：247 / 252

页数：6

共 22 条

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