HIV ANTIGEN-DETECTION IN CIRCULATING IMMUNE-COMPLEXES

被引:33
作者
KESTENS, L [1 ]
HOOFD, G [1 ]
GIGASE, PL [1 ]
DELEYS, R [1 ]
VANDERGROEN, G [1 ]
机构
[1] INNOGENET NV,ANTWERP,BELGIUM
关键词
AIDS; HIV; IMMUNE COMPLEX; ANTIGEN DETECTION; IMMUNE COMPLEX DISSOCIATION;
D O I
10.1016/0166-0934(91)90145-P
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Various methods were evaluated for their effectiveness in releasing HIV antigen (Ag) from artificial immune complexes (IC) and from IC present in serum from HIV antibody (Ab) positive subjects. The most effective methods for recovering HIV Ag from IC were those which included a denaturation step to prevent reassociation of Ag with Ab. IC precipitation in 2.5% polyethylene glycol followed by acid treatment with 1 M glycine.HCl (pH 2) for 10 min at 70-degrees-C in the presence of 0.05% SDS gave very satisfactory results. With this method, IC were detected in sera from HIV antibody positive Caucasian subjects at all stages of infection. After HIV IC dissociation, HIV Ag was detected in a significant number (8/17 or 47%) of asymptomatic subjects. IC were most prevalent during the late stages of infection. A substantial increase in HIV Ag positivity was also observed in 20 Senegalese HIV Ab positive sera. After HIV IC dissociation HIV antigen detection increased from 2/20 to 12/20. The relevance of IC detection is discussed.
引用
收藏
页码:67 / 76
页数:10
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