AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION AND QUANTITATION OF THE TUMOR-MARKER 1-METHYLINOSINE IN HUMAN URINE

被引：12

作者：

DAMBROSIO, SM

GIBSONDAMBROSIO, RE

TREWYN, RW

机构：

[1] OHIO STATE UNIV, CTR COMPREHENS CANC, COLUMBUS, OH 43210 USA

[2] OHIO STATE UNIV, DEPT PHYSIOL CHEM, COLUMBUS, OH 43210 USA

来源：

CLINICA CHIMICA ACTA | 1991年 / 199卷 / 02期

关键词：

TUMOR MARKER; ELISA; NUCLEOSIDE; URINE; HUMAN; 1-METHYLINOSINE;

D O I：

10.1016/0009-8981(91)90103-J

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A highly sensitive enzyme-linked immunoassay (ELISA) was developed to detect and quantify the tumor marker, 1-methylinosine (m1I), in human urine. The rabbit antisera was highly specific for m1I with negligible or no inhibition by other nucleosides excreted into urine. Using the competitive ELISA, nanogram amounts of m1I were easily measured directly in urine. The assay agreed with our previous hplc analysis of m1I in urine for identifying those individuals with chronic myelogenous leukemia. Thus, this assay should greatly facilitate the quantitation of m1I as a tumor marker.

引用

页码：119 / 128

页数：10

共 19 条

[1]

BOREK E, 1986, CANCER RES, V46, P2557

[2]

BOREK E, 1983, CANCER DETECT PREV, V6, P67

[3]

GEHRKE CW, 1979, CANCER RES, V39, P1150

[4]

HELDMAN DA, 1983, J LAB CLIN MED, V101, P783

[5]

HELDMAN DA, 1983, BLOOD, V61, P291

[6]

HELDMAN DA, 1983, J NATL CANCER I, V71, P269

[7] DETECTION OF ELEVATED AMOUNTS OF URINARY PSEUDOURIDINE IN CANCER-PATIENTS BY USE OF A MONOCLONAL-ANTIBODY [J].

ITOH, K ;

MIZUGAKI, M ;

ISHIDA, N .

CLINICA CHIMICA ACTA, 1989, 181 (03) :305-315

[8]

Johnson D. A., 1984, Gene Analysis Techniques, V1, P3, DOI 10.1016/0735-0651(84)90049-9

[9] URINARY MODIFIED NUCLEOSIDES AS TUMOR-MARKERS IN CANCER OF THE URINARY ORGANS OR FEMALE GENITAL-TRACT [J].

KOSHIDA, K ;

HARMENBERG, J ;

STENDAHL, U ;

WAHREN, B ;

BORGSTROM, E ;

HELSTROM, L ;

ANDERSSON, L .

UROLOGICAL RESEARCH, 1985, 13 (05) :213-218

[10]

LEVINE L, 1975, JNCI-J NATL CANCER I, V54, P341

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