PURIFICATION OF ACETYL-COA - 17-O-DEACETYLVINDOLINE 17-O-ACETYLTRANSFERASE FROM CATHARANTHUS-ROSEUS LEAVES

被引：10

作者：

FAHN, W

STOCKIGT, J

机构：

[1] Institut für Pharmazeutische Biologie, Universität München, München 2, D-8000

来源：

PLANT CELL REPORTS | 1990年 / 8卷 / 10期

关键词：

D O I：

10.1007/BF00270066

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The enzyme acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyltransferase which terminates vindoline biosynthesis has been isolated from Catharanthus roseus leaves, further characterized and purified to homogeneity by three step column chromatography and subsequent preparative isoelectric focusing. Kinetic properties concerning the enzyme reaction are discussed. Five multiple forms of the acetyl-transferase could be observed, each consisting of two subunits. This enzyme is now the best characterized of the enzymes involved in vindoline biosynthesis. © 1990 Springer-Verlag.

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页码：613 / 616

页数：4

相关论文

共 16 条

[1] AN IMPROVEMENT OF THE PROTEIN DETERMINATION IN PLANT-TISSUES WITH THE DYE-BINDING METHOD ACCORDING TO BRADFORD [J].

APPENROTH, KJ ;

AUGSTEN, H .

BIOCHEMIE UND PHYSIOLOGIE DER PFLANZEN, 1987, 182 (01) :85-89

[2]

ATKINSON T, 1981, Biochemical Society Transactions, V9, P290

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

CLELAND WW, 1977, ADV ENZYMOLOGY, V45, P287

[5] DEVELOPMENTAL REGULATION OF ENZYMES OF INDOLE ALKALOID BIOSYNTHESIS IN CATHARANTHUS-ROSEUS [J].

DELUCA, V ;

FERNANDEZ, JA ;

CAMPBELL, D ;

KURZ, WGW .

PLANT PHYSIOLOGY, 1988, 86 (02) :447-450

[6] SUBCELLULAR-LOCALIZATION OF ENZYMES INVOLVED IN INDOLE ALKALOID BIOSYNTHESIS IN CATHARANTHUS-ROSEUS [J].

DELUCA, V ;

CUTLER, AJ .

PLANT PHYSIOLOGY, 1987, 85 (04) :1099-1102

[7]

DELUCA V, 1987, PLANT CELL REP, V6, P458, DOI 10.1007/BF00272782

[8] ACETYL COENZYME-A - DEACETYLVINDOLINE O-ACETYLTRANSFERASE, A NOVEL ENZYME FROM CATHARANTHUS [J].

DELUCA, V ;

BALSEVICH, J ;

KURZ, WGW .

JOURNAL OF PLANT PHYSIOLOGY, 1985, 121 (05) :417-428

[9] BIOSYNTHESIS OF INDOLE ALKALOIDS - DEVELOPMENTAL REGULATION OF THE BIOSYNTHETIC-PATHWAY FROM TABERSONINE TO VINDOLINE IN CATHARANTHUS-ROSEUS [J].

DELUCA, V ;

BALSEVICH, J ;

TYLER, RT ;

EILERT, U ;

PANCHUK, BD ;

KURZ, WGW .

JOURNAL OF PLANT PHYSIOLOGY, 1986, 125 (1-2) :147-156

[10] LATE ENZYMES OF VINDOLINE BIOSYNTHESIS - S-ADENOSYL-L-METHIONINE-11-O-DEMETHYL-17-O-DEACETYLVINDOLINE 11-O-METHYLTRANSFERASE AND UNSPECIFIC ACETYLESTERASE [J].

FAHN, W ;

LAUSSERMAIR, E ;

NEUMANN, BD ;

STOCKIGT, J .

PLANT CELL REPORTS, 1985, 4 (06) :337-340