A SIMPLE SYNTHESIS OF OCTYL 3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-BETA-D-MANNOPYRANOSIDE AND ITS USE AS AN ACCEPTOR FOR THE ASSAY OF N-ACETYLGLUCOSAMINYLTRANSFERASE-I ACTIVITY

被引：48

作者：

KAUR, KJ ^{[1
]}

HINDSGAUL, O ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

GLYCOCONJUGATE JOURNAL | 1991年 / 8卷 / 02期

关键词：

N-ACETYLGLUCOSAMINYLTRANSFERASE-I; GLYCOSYLTRANSFERASE ASSAY; CORE-TRIMANNOSIDE; OLIGOSACCHARIDE SYNTHESIS;

D O I：

10.1007/BF00731017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple synthesis of octyl 3,6-di-O-(alpha-D-mannopyranosyl)-beta-D-mannopyranoside is described. The key features of the synthetic scheme are the formation of the beta-mannosidic linkage by 1-O-alkylation of 2,3,4,6-tetra-O-acetyl-alpha,beta-D-mannopyranose with octyl iodide and glycosylation of unprotected octyl beta-D-mannopyranoside using limiting acetobromomannose. The trisaccharide is shown to be an acceptor for N-acetylglucosaminyltransferase-I with a K(M) of 585-mu-M.

引用

页码：90 / 94

页数：5

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