ISOLATION OF MONOCLONAL-ANTIBODIES THAT REACT PREFERENTIALLY WITH LISTERIA-MONOCYTOGENES

被引：8

作者：

GAVALCHIN, J ^{[1
]}

TORTORELLO, ML ^{[1
]}

MALEK, R ^{[1
]}

LANDERS, M ^{[1
]}

BATT, CA ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT FOOD SCI,107 STOCKING HALL,ITHACA,NY 14853

来源：

FOOD MICROBIOLOGY | 1991年 / 8卷 / 04期

关键词：

D O I：

10.1016/S0740-0020(05)80006-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have isolated and characterized three monoclonal antibodies that preferentially recognize the pathogen Listeria monocytogenes. Mouse monoclonal antibodies were produced by fusion of spleen cells from a Balb/c mouse, which was immunized with live L. monocytogenes, to an NS-1 myeloma partner. Hybridomas that produced antibodies which preferentially reacted with L. monocytogenes were identified by a direct binding enzyme-linked immunoabsorbent assay (ELISA) using a panel of bacteria which included L. ivanovii, Streptococcus faecalis, L. seeligeri, L. innocua and L. monocytogenes Scott A. Three hybridomas, Mab 20-10-2, Mab 36-6-12 and Mab 56-9-16 which preferentially reacted with L. monocytogenes were selected for further analysis. An indirect ‘sandwich’ ELISA was developed and used to further confirm that these hybridomas reacted with a number of L. monocytogenes isolates and not with other common cross-reacting bacteria including Kurthia sp. © 1991, Academic Press Limited. All rights reserved.

引用

页码：325 / 330

页数：6

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