SPECIFICITY OF FACTOR-XA IN THE CLEAVAGE OF FUSION PROTEINS

被引：25

作者：

HE, MG ^{[1
]}

JIN, L ^{[1
]}

AUSTEN, B ^{[1
]}

机构：

[1] ST GEORGE HOSP, SCH MED, DEPT SURG, PEPTIDE UNIT, LONDON SW17 0RE, ENGLAND

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1993年 / 12卷 / 01期

基金：

英国惠康基金;

关键词：

FACTOR-XA; FUSION PROTEINS; BACTERIAL EXPRESSION; PROTEOLYTIC SPECIFICITY;

D O I：

10.1007/BF01024906

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The precursor protein honey bee prepromelittin has been expressed as a fusion protein in Escherichia coli joined to the C-terminus of a truncated form of the bacteriophage gene 10 protein via an engineered recognition sequence for Factor Xa. Factor Xa was found to cleave poorly at the engineered site, giving a low yield of the required prepromelittin. In contrast, cleavage on the C-terminal side of the sequence VLGR at residue 67 in the gene 10 sequence proceeded in high yield. Factor Xa may be inhibited by adjacent hydrophobic sequences on the C-terminal side of a potential cleavage site.

引用

页码：1 / 5

页数：5

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