SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR PRAZIQUANTEL IN PLASMA, URINE AND LIVER HOMOGENATES

被引：15

作者：

GONZALEZESQUIVEL, DF ^{[1
]}

OKUNO, CM ^{[1
]}

RODRIGUEZ, MS ^{[1
]}

MORALES, JS ^{[1
]}

COOK, HJ ^{[1
]}

机构：

[1] NATL INST NEUROL & NEUROSURG,NEUROPHARMACOL & NEUROIMMUNOL LABS,MEXICO CITY,MEXICO

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80213-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of praziquantel in plasma, urine and rat liver homogenates has been developed. It requires 2 ml of biological fluid, an extraction using Sep-Pak cartridges, a 0.05 M phosphate buffer solution (pH 5.0) for equilibrating and washing and ethyl acetate-diisopropyl ether for drug elution. The analysis was performed on an Ultrasphere ODS C18 column with a mobile phase of acetonitrile-water with ultraviolet detection at 217 nm. The results showed that the assay is sensitive (31.2 ng/ml), linear between 0.125 and 4.0 mug/ml, precise (coefficient of variation = 10%) and selective with other drugs currently administered with praziquantel.

引用

页码：174 / 178

页数：5

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