LOCALIZATION OF CYCLIN-A AT THE SITES OF CELLULAR DNA-REPLICATION

被引：108

作者：

SOBCZAKTHEPOT, J ^{[1
]}

HARPER, F ^{[1
]}

FLORENTIN, Y ^{[1
]}

ZINDY, F ^{[1
]}

BRECHOT, C ^{[1
]}

PUVION, E ^{[1
]}

机构：

[1] IRSC,BIOL ULTRASTRUCT NOYAU LAB,CNRS,UPR 272,F-94801 VILLEJUIF,FRANCE

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 206卷 / 01期

关键词：

D O I：

10.1006/excr.1993.1118

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cyclin A is a nuclear protein which is part of a kinase complex with either p34cdc2 or p33cdk2. Cyclin A is required in higher eukaryotic cells at the G1/S and the G2/M transitions. To examine the relationship between cyclin A and DNA replication, we simultaneously labeled exponentially growing HeLa cells for the distribution of cyclin A and proliferating cell nuclear antigen (PCNA). We have now demonstrated, by means of immunoelectron microscopy, that cyclin A is located at the sites of DNA replication visualized by both BrdU and PCNA labeling. Thus cyclin A may play a significant role in the phosphorylation of proteins at or near the sites of DNA replication. © 1993 Academic Press, Inc.

引用

页码：43 / 48

页数：6

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