HUMAN CUZN SUPEROXIDE-DISMUTASE ENZYMATIC-ACTIVITY IN CELLS IS REGULATED BY THE LENGTH OF THE MESSENGER-RNA

被引：12

作者：

KILK, A ^{[1
]}

LAAN, M ^{[1
]}

TORP, A ^{[1
]}

机构：

[1] ESTONIAN ACAD SCI,INST CHEM PHYS & BIOPHYS,TARTU 2400,ESTONIA

来源：

FEBS LETTERS | 1995年 / 362卷 / 03期

关键词：

CUZNSOD ACTIVITY; SOD CDNA; 3'-UTR;

D O I：

10.1016/0014-5793(95)00266-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single functional human CuZnSOD gene encodes two species of mRNA differing in size by 200 nucleotides in the 3'-untranslated region (UTR). We studied the expression of the CuZnSOD cDNA with different 3'- and 5'-UTR. Deletion in the 5'-end does not affect the expression of the enzyme, however, deletion in the 3'-UTR decreases the level of expression of CuZnSOD. The plasmids containing the long CuZnSOD cDNA with all polyadenylation signal sequences utilize primarily the last polyadenylation site and give a long mRNA, which produces three times more enzyme than the short mRNA lacking the last polyadenylation site and the AU-rich region.

引用

页码：323 / 327

页数：5

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