USE OF NEW PHOSPHONYLATING AND COUPLING AGENTS IN THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES VIA THE H-PHOSPHONATE APPROACH

被引：15

作者：

SAKATSUME, O

YAMANE, H

TAKAKU, H

YAMAMOTO, N

机构：

[1] CHIBA INST TECHNOL,DEPT IND CHEM,BIOORGAN CHEM LAB,NARASHINO,CHIBA 275,JAPAN

[2] YAMAGUCHI UNIV MED,DEPT VIROL & PARASITOL,UBE 755,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 11期

关键词：

D O I：

10.1093/nar/18.11.3327

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New phosphonylating and coupling agents for the synthesis of oligodeoxyribonucleotides via Hphosphonate approach have been developed. Tris(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite, prepared by the reaction of lithium salt of 1,1,1,3,3,3-hexafluoro-2-propoxide with PCI3, reacts with deoxyribonucleosides in the presence of a catalytic amount of triethylamine to produce in the high yield the corresponding deoxyribonucleoside 3′- phosphonate units. The use of a new coupling reagent, 1,3-dimethyl-2-chloro-imidazolinium chloride (DMCI) for the internucleotidic H-phosphonate bond formation via the H-phosphonate approach is also discussed in detail. © 1990 Oxford University Press.

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页码：3327 / 3331

页数：5

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