IDENTIFICATION OF B2 BRADYKININ BINDING-SITES ON CULTURED CORTICAL ASTROCYTES

被引：47

作者：

CHOLEWINSKI, AJ ^{[1
]}

STEVENS, G ^{[1
]}

MCDERMOTT, AM ^{[1
]}

WILKIN, GP ^{[1
]}

机构：

[1] IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 57卷 / 04期

关键词：

BRADYKININ; BRADYKININ RECEPTORS; ASTROCYTES; CELL CULTURE;

D O I：

10.1111/j.1471-4159.1991.tb08314.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single K(D) of 16.6 +/- 2.6 nM and a B(max) of 352.2 +/- 30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.

引用

页码：1456 / 1458

页数：3

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