A CONSENSUS ALU REPEAT PROBE FOR PHYSICAL MAPPING

被引：33

作者：

BATZER, MA

ALEGRIAHARTMAN, M

DEININGER, PL

机构：

[1] LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,NEW ORLEANS,LA

[2] ALTON OCHSNER MED FDN & OCHSNER CLIN,MOLEC GENET LAB,NEW ORLEANS,LA 70121

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1994年 / 11卷 / 02期

关键词：

D O I：

10.1016/1050-3862(94)90058-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to black the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here, we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes.

引用

页码：34 / 38

页数：5

共 17 条

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