STUDIES INTO THE MICROBIAL SPECTRUM OF APICAL PERIODONTITIS

被引：17

作者：

BRAUNER, AW

CONRADS, G

机构：

[1] Clinic of Conservative and Preventive Dentistry & Periodontology, Technical University (RWTH), Aachen, 52057

[2] Institute of Medical Microbiology, Technical University (RWTH), Aachen, 52057

来源：

INTERNATIONAL ENDODONTIC JOURNAL | 1995年 / 28卷 / 05期

关键词：

DNA PROBES; MICROBIOLOGY; PERIAPICAL GRANULOMA SAMPLES; ROOT CANAL SAMPLES; SPECTRUM OF BACTERIA;

D O I：

10.1111/j.1365-2591.1995.tb00308.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. A total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort, Bacteria were identified by applying the following techniques: culturing on various media, Gram-staining and using commercially available biochemical test strips, In addition, Prevotella intermedia and Porphyromonas endodontalis were differentiated on a molecular genetic level using species-specific oligodeoxynucleotide probes. The most frequently identified bacteria were Prevotella intermedia, Bifidobacterium spp,, Streptococcus sanguis, Streptococcus milleri-group and Bacteroides spp, Obligate anaerobes occured at a rate of 82.3%, and the average number of isolates was 6.4 per sample.

引用

页码：244 / 248

页数：5

共 20 条

[1] Auerhold L, Knothe H, Frenkel G, The bacteriology of dentogenous pyogenic infections, Oral Surgery, Oral Medicine, Oral Pathology, 52, pp. 583-587, (1981)
[2] Balimgartner JC, Falkler W, Bacteria in the apical 5 mm of infected root canals, Journal of Endodontics, 17, pp. 380-383, (1991)
[3] Bekgenholtz G, Microorganisms from necrotic pulp of traumatized teeth, Qndantolagical Reviews, 25, pp. 347-348, (1976)
[4] Brook J, Frazier EH, Aerobic and anaerobic microbiology of periapical abscess, Oral Microbiology and Immunology, 6, 2, pp. 123-125, (1991)
[5] Conkads G, Brauner A, Non‐radioactively labdled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas. species, FEMS Immunology & Medical Microbiology, 6, pp. 115-120, (1993)
[6] Engstrom B, Frostell G, Bacteriological studies of the non‐vital pulp in cases with intact pulp cavities, Actu Qdontaloyica Scandinavia, 19, pp. 23-39, (1961)
[7] Griffeb MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW, The relationship of Bacteroides melaninogaticus to symptoms associated with pulpal necrosis, Oral Surgery, Oral Medicine, Oral Pathology, 50, pp. 457-461, (1980)
[8] Haapasalo M, Bacteroides buccae and related taxa in necrotic root canal infections, Journal of Clinical Microbiology, 26, 6, pp. 940-944, (1986)
[9] Head J, Roos C, On the bacteriology of apical abscesses, Journal of Dental Research, 1, (1919)
[10] Hillmann JD, Socransky SS, Shavers M, The relationship between streptococcal species and periodontopathic bacteria in human dental plaque, Archives of Oral Biology, 30, pp. 791-795, (1985)

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