BACTERIAL-DEGRADATION OF M-NITROBENZOIC ACID

被引：34

作者：

NADEAU, LJ ^{[1
]}

SPAIN, JC ^{[1
]}

机构：

[1] ARMSTRONG LAB,EQC OL,TYNDALL AFB,FL 32403

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 02期

关键词：

D O I：

10.1128/AEM.61.2.840-843.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pseudomonas sp. strain JS51 grows on m-nitrobenzoate (m-NBA) with stoichiometric release of nitrite, m-NBA-grown cells oxidized m-NBA and protocatechuate but not 3-hydroxybenzoate, 4-hydroxy-3-nitrobenzoate, 4-nitrocatechol, and 1,2,4-benzenetriol. Protocatechuate accumulated transiently when succinate-grown cells were transferred to media containing m-NBA. Respirometric experiments indicated that the conversion of m-NBA to protocatechuate required 1 mol of oxygen per mol of substrate. Conversions conducted in the presence of O-18(2) showed the incorporation of both atoms of molecular oxygen into protocatechuate. Extracts of m-NBA-grown cells cleaved protocatechuate to 2-hydroxy-4-carboxymuconic semialdehyde. These results provide rigorous proof that m-NBA is initially oxidized by a dioxygenase to produce protocatechuate which is further degraded by a 4,5-dioxygenase.

引用

页码：840 / 843

页数：4

共 28 条

[1]

ARCIERO DM, 1990, METHOD ENZYMOL, V188, P89

[2]

Bayly R.C., 1984, MICROBIOL, V3, P253

[3] CATABOLISM OF NAPHTHALENESULFONIC ACIDS BY PSEUDOMONAS SP A3 AND PSEUDOMONAS SP C22 [J].