REPRODUCTIVE CAPACITY OF THE NUCLEUS OF THE MALE GAMETE AFTER COMPLETION OF MEIOSIS

被引:103
作者
SOFIKITIS, NV [1 ]
MIYAGAWA, I [1 ]
AGAPITOS, E [1 ]
PASYIANOS, P [1 ]
TODA, T [1 ]
HELLSTROM, WJG [1 ]
KAWAMURA, H [1 ]
机构
[1] TOTTORI UNIV,SCH MED,DEPT UROL,YONAGO,TOTTORI 683,JAPAN
关键词
FERTILITY; RABBIT; ROUND SPERMATID; LASER MICROSCOPE;
D O I
10.1007/BF02214138
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Purpose: Our purpose was to investigate the possibility of achieving fertilization and subsequent nor mal embryonic development by injecting round spermatid nuclei into rabbit oocytes. Results: Two- to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop in vitro up to the expanding blastocyst stage or in vivo lip to complete gestation. Conclusion: The current findings show that the haploid set of chromosomes of round spermatid can pair with the chromosomes of the ootid to participate in complete fertilization and subsequent embryonic and fetal development. In addition, we suggest that postmeiotic modifications of the round spermatid are not required for the pairing of male gamete chromosomes with those of the ootid.
引用
收藏
页码:335 / 341
页数:7
相关论文
共 19 条
  • [1] De Kretser D.M., Kerr J.B., The cytology of the testis, The Physiology of Reproduction, pp. 837-8667, (1988)
  • [2] Fishel S., Historical overview of the use of micromanipulation, Gamete and Embryo Micromanipulation in Human Reproduction, pp. 3-17, (1993)
  • [3] Mc Grath J., Solter D., Maternal lethality in the mouse is a nuclear, not cytoplasmic defect, Nature, 308, pp. 550-551, (1894)
  • [4] Renard J.P., Babinet C., Identification of a paternal developmental effect on the cytoplasm of 1-cell stage mouse embryos, Proc Natl Acad Sci USA, 83, pp. 6883-6886, (1986)
  • [5] Ng S.C., Solter D., Fusion of male germ cells from male pronucleus to pachytene spermatocytes with metaphase II oocytes in mice, Mol Androl, 4, pp. 264-276, (1992)
  • [6] Ogura A., Yanagimachi R., Round spermatid nuclei injected into hamster oocytes form pronuclei and participate in syngamy, Biol Reprod, 48, pp. 219-225, (1993)
  • [7] Li J., Foote R.H., Simkin M., Development of rabbit zygotes cultured in protein-free medium with catalase, taurine, or Superoxide dismutase, Biol Reprod, 48, pp. 33-37, (1993)
  • [8] Carney E.W., Foote R.H., Improved development of rabbit one-cell embryos to the hatching blastocyst stage by culture in a defined protein-free culture medium, J Reprod Fertil, 91, pp. 113-223, (1991)
  • [9] Al-Hasani S., Trotnow S., Sadtler C., Hahn J., In vitro fertilization and embryo transfer of pre-ovulatory rabbit oocytes, European Journal of Obstetrics & Gynecology and Reproductive Biology, 21, pp. 187-195, (1986)
  • [10] Brackett B.G., Oliphant G., Capacitation of rabbit spermatozoa in vitro, Biol Reprod, 12, pp. 260-274, (1975)