DETERMINATION OF EPITESTOSTERONE AND TESTOSTERONE IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：29

作者：

NAVAJAS, R

IMAZ, C

CARRERAS, D

GARCIA, M

PEREZ, M

RODRIGUEZ, C

RODRIGUEZ, AF

CORTES, R

机构：

[1] Laboratorio de Control de Dopaje, Consejo Superior de Deportes, 28040 Madrid, c/ Greco s/n

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 673卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00260-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C-18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.

引用

页码：159 / 164

页数：6

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