FACTOR-XA CLEAVAGE OF FUSION PROTEINS - ELIMINATION OF NONSPECIFIC CLEAVAGE BY REVERSIBLE ACYLATION

被引：27

作者：

WEARNE, SJ

机构：

[1] MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, Hills Road

来源：

FEBS LETTERS | 1990年 / 263卷 / 01期

关键词：

Factor Xa; Fusion protein cleavage; Protein modification;

D O I：

10.1016/0014-5793(90)80696-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the elimination of non-specific cleavage of fusion proteins by factor Xa. Putative non-specific cleavage sites C-terminal to lysyl residues are blocked by reversible acylation by 3,4,5,6-tetrahydrophthalic anhydride prior to cleavage. After cleavage, the acyi groups are removed quantitatively by exposure to slightly acidic conditions. This method employs no harsh reagents or conditions, and may be generally applicable to factor Xa cleavage of fusion proteins. © 1990.

引用

页码：23 / 26

页数：4

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