RAPID RESTRICTION MAPPING OF DNA CLONED IN LAMBDA PHAGE VECTORS

A protocol for the rapid restriction mapping of phage .lambda. clones was developed. Partial digestion products are selectively labeled at the right or left cohesive .lambda. DNA termini by hybridization with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the ladder of partial digestion products.