EXPRESSION OF STAPHYLOCOCCAL-ENTEROTOXIN C-1 IN ESCHERICHIA-COLI

被引:30
作者
BOHACH, GA [1 ]
SCHLIEVERT, PM [1 ]
机构
[1] UNIV MINNESOTA, SCH MED, DEPT MICROBIOL, MINNEAPOLIS, MN 55455 USA
关键词
D O I
10.1128/IAI.55.2.428-432.1987
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The structural gene encoding staphylococcal enterotoxin C1 was cloned into Escherichia coli and localized on a 1.5-kilobase HindIII-ClaI DNA fragment by subcloning. The toxin was partially purified from E. coli clones and shown to be immunologically identical to enterotoxin C1 from Staphylococcus aureus. The cloned toxin also had the same molecular weight (26,000) and charge heterogeneity as staphylococcus-derived enterotoxin. Toxins from both sources were equally biologically active.
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页码:428 / 432
页数:5
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