AN ACTIN-RELATED PROTEIN FROM DICTYOSTELIUM-DISCOIDEUM IS DEVELOPMENTALLY-REGULATED AND ASSOCIATED WITH MITOCHONDRIA

被引：22

作者：

MURGIA, I ^{[1
]}

MACIVER, SK ^{[1
]}

MORANDINI, P ^{[1
]}

机构：

[1] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND

来源：

FEBS LETTERS | 1995年 / 360卷 / 03期

关键词：

ACTIN-LIKE PROTEIN; CDNA SEQUENCE; NORTHERN BLOT ANALYSIS; IMMUNOFLUORESCENCE; ARP3; MITOCHONDRION;

D O I：

10.1016/0014-5793(95)00111-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An actin-related protein (ACLA) has been identified in the cellular slime mould Dictyostelium discoideum. The complete cDNA sequence indicates that within the actin superfamily it belongs to the ARP3 family of actin-related proteins together with Arp66B from Drosophila melanogaster, Actin2 from Bos taurus, act2 from Schizosaccharomyces pombe and possibly act2 from Caenorhabditis elegans. The ACLA mRNA is regulated during development, showing a maximum between 2 and 4 h after starvation. The protein has been expressed in E. coli and antibodies raised against it. Immunofluorescence microscopy shows that ACLA protein co-localises with mitochondria; the protein copurifies with Dictyostelium mitochondria.

引用

页码：235 / 241

页数：7

共 46 条

[1]

BERKS M, 1990, DEVELOPMENT, V110, P977

[2] CYTOCHROME-C-OXIDASE FROM THE SLIME-MOLD DICTYOSTELIUM-DISCOIDEUM - PURIFICATION AND CHARACTERIZATION [J].