MACROMOLECULAR RECOGNITION THROUGH ELECTROSTATIC REPULSION

被引:14
作者
BEDOUELLE, H
NAGEOTTE, R
机构
[1] Grp. d'Ingenierie des Proteines, Unité de Biochimie Cellulaire, Institut Pasteur, 75724 Paris Cedex 15
关键词
AMINOACYL-TRANSFER-RNA SYNTHETASE; DISCRIMINATION; SPECIFICIT; /TRANSFER RNA; TYROSYL-TRANSFER-RNA SYNTHETASE;
D O I
10.1002/j.1460-2075.1995.tb07294.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the process of genetic translation, each aminoacyl-tRNA synthetase specifically aminoacylates its cognate tRNAs and rejects the 19 other species of tRNAs, A decrease in the specificity of this reaction can result in misincorporations of amino acids into proteins and be deleterious to the cell, In the case of tyrosyl-tRNA synthetase from Bacillus stearothermophilus, the change of residue Glu152 into Ala results in erroneous interactions with non-cognate tRNAs. To analyse how Glu152 contributes to the discrimination between tRNAs by tyrosyl-tRNA synthetase, 11 changes to this residue were created by mutagenesis. The misaminoacylations of tRNA(Phe) and tRNA(Val) with tyrosine in vitro (on a scale going from 1 to 30) and the toxicity of tyrosyl-tRNA synthetase in vivo (on a scale from 1 to 10(7)) increased in a correlated way when the nature of the side chain in position 152 varied from negatively charged to uncharged then to positively charged, The aminoacylation of tRNA(Tyr) was unaffected by the mutations. The results show that the role of Glu152 in the discrimination between tRNAs is purely negative, that it acts by electrostatic repulsion of non-cognate tRNAs and that this mechanism has been conserved throughout evolution.
引用
收藏
页码:2945 / 2950
页数:6
相关论文
共 34 条
  • [1] USE OF BINDING-ENERGY IN CATALYSIS - OPTIMIZATION OF RATE IN A MULTISTEP REACTION
    AVIS, JM
    FERSHT, AR
    [J]. BIOCHEMISTRY, 1993, 32 (20) : 5321 - 5326
  • [2] DISCRIMINATION BETWEEN TRANSFER-RNAS BY TYROSYL-TRANSFER-RNA SYNTHETASE
    BEDOUELLE, H
    GUEZIVANIER, V
    NAGEOTTE, R
    [J]. BIOCHIMIE, 1993, 75 (12) : 1099 - 1108
  • [3] A MODEL OF SYNTHETASE TRANSFER-RNA INTERACTION AS DEDUCED BY PROTEIN ENGINEERING
    BEDOUELLE, H
    WINTER, G
    [J]. NATURE, 1986, 320 (6060) : 371 - 373
  • [4] RECOGNITION OF TRANSFER RNATYR BY TYROSYL-TRANSFER RNA-SYNTHETASE
    BEDOUELLE, H
    [J]. BIOCHIMIE, 1990, 72 (08) : 589 - 598
  • [5] OVERPRODUCTION OF TYROSYL-TRANSFER RNA-SYNTHETASE IS TOXIC TO ESCHERICHIA-COLI - A GENETIC-ANALYSIS
    BEDOUELLE, H
    GUEZ, V
    VIDALCROS, A
    HERMANN, M
    [J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (07) : 3940 - 3945
  • [6] THE 2.9 ANGSTROM CRYSTAL-STRUCTURE OF THERMUS-THERMOPHILUS SERYL-TRANSFER-RNA SYNTHETASE COMPLEXED WITH TRNA(SER)
    BIOU, V
    YAREMCHUK, A
    TUKALO, M
    CUSACK, S
    [J]. SCIENCE, 1994, 263 (5152) : 1404 - 1410
  • [7] STRUCTURE OF TYROSYL TRANSFER-RNA SYNTHETASE REFINED AT 2.3-A RESOLUTION - INTERACTION OF THE ENZYME WITH THE TYROSYL ADENYLATE INTERMEDIATE
    BRICK, P
    BHAT, TN
    BLOW, DM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1989, 208 (01) : 83 - 98
  • [8] CAINAN BJ, 1991, SCIENCE, V252, P1167
  • [9] PURIFICATION AND PHYSICAL CHARACTERIZATION OF TYROSYL RIBONUCLEIC ACID SYNTHETASES FROM ESCHERICHIA COLI AND BACILLUS SUBTILIS
    CALENDAR, R
    BERG, P
    [J]. BIOCHEMISTRY, 1966, 5 (05) : 1681 - &
  • [10] THE ACTIVE-SITE OF YEAST ASPARTYL-TRANSFER-RNA SYNTHETASE - STRUCTURAL AND FUNCTIONAL-ASPECTS OF THE AMINOACYLATION REACTION
    CAVARELLI, J
    ERIANI, G
    REES, B
    RUFF, M
    BOEGLIN, M
    MITSCHLER, A
    MARTIN, F
    GANGLOFF, J
    THIERRY, JC
    MORAS, D
    [J]. EMBO JOURNAL, 1994, 13 (02) : 327 - 337