VIABILITY OF THE NEONATAL RAT ISOLATED BRAIN-STEM PREPARATION BY P-31 MRS

被引：4

作者：

BURTON, MD ^{[1
]}

HITZIG, BM ^{[1
]}

JOHNSON, DC ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,BOSTON,MA 02115

来源：

NEUROSCIENCE LETTERS | 1994年 / 169卷 / 1-2期

关键词：

ISOLATED BRAIN-STEM; VIABILITY; NMR SPECTROSCOPY; PHOSPHATE; INTRACELLULAR PH;

D O I：

10.1016/0304-3940(94)90349-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The isolated brainstem-spinal axis from the neonatal rat is an established model for studying neuronal responses of the ventilatory control system, however, its viability has not been clearly established. We studied the brainstem-spinal axis from newborn rats at 8.5 T with P-31 NMR spectroscopy. The relative pattern of high energy phosphates (HEPs) was similar to that reported for the in vivo neonatal brain. The average pH(i) was 0.2 to 0.4 units less than the pH(i) for the in vivo neonatal brain. The HEPs and pH(i) were stable for 6 h, suggesting extended in vitro viability.

引用

页码：31 / 34

页数：4

共 14 条

[1] CORRELATION BETWEEN P-31 NMR PHOSPHOMONOESTER AND BIOCHEMICALLY DETERMINED PHOSPHORYLETHANOLAMINE AND PHOSPHATIDYLETHANOLAMINE DURING DEVELOPMENT OF THE RAT-BRAIN
BURRI, R
LAZEYRAS, F
AUE, WP
STRAEHL, P
BIGLER, P
ALTHAUS, U
HERSCHKOWITZ, N
[J]. DEVELOPMENTAL NEUROSCIENCE, 1988, 10 (04) : 213 - 221
[2] CADY EB, 1983, LANCET, V1, P1059
[3] ACID HOMEOSTASIS FOLLOWING PARTIAL ISCHEMIA IN NEONATAL BRAIN MEASURED INVIVO BY P-31 AND H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY
CORBETT, RJT
LAPTOOK, AR
[J]. JOURNAL OF NEUROCHEMISTRY, 1990, 54 (04) : 1208 - 1217
[4] INTRACELLULAR PH, LACTATE, AND ENERGY-METABOLISM IN NEONATAL BRAIN DURING PARTIAL ISCHEMIA MEASURED INVIVO BY P-31 AND H-1 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
CORBETT, RJT
LAPTOOK, AR
NUNNALLY, RL
HASSAN, A
JACKSON, J
[J]. JOURNAL OF NEUROCHEMISTRY, 1988, 51 (05) : 1501 - 1509
[5] PHOSPHORYLETHANOLAMINE - THE MAJOR CONSTITUENT OF THE PHOSPHOMONOESTER PEAK OBSERVED BY P-31-NMR ON DEVELOPING DOG BRAIN
GYULAI, L
BOLINGER, L
LEIGH, JS
BARLOW, C
CHANCE, B
[J]. FEBS LETTERS, 1984, 178 (01) : 137 - 142
[6] IDENTIFICATION OF A SUBSURFACE AREA IN THE VENTRAL MEDULLA SENSITIVE TO LOCAL CHANGES IN PCO2
ISSA, FG
REMMERS, JE
[J]. JOURNAL OF APPLIED PHYSIOLOGY, 1992, 72 (02) : 439 - 446
[7] EFFECTS OF HYPOTHERMIA ON RAT-BRAIN PHI AND PHOSPHATE METABOLITE REGULATION BY P-31-NMR
JOHNSON, DC
NISHIMURA, M
OKUNIEFF, P
KAZEMI, H
HITZIG, B
[J]. JOURNAL OF APPLIED PHYSIOLOGY, 1989, 67 (06) : 2527 - 2534
[8] AUTORADIOGRAPHIC DETERMINATION OF BRAIN PH FOLLOWING MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE RAT
KOBATAKE, K
SAKO, K
IZAWA, M
YAMAMOTO, YL
HAKIM, AM
[J]. STROKE, 1984, 15 (03) : 540 - 547
[9] PH STANDARDIZATION FOR P-31 MAGNETIC-RESONANCE HEART SPECTROSCOPY AT DIFFERENT TEMPERATURES
KOST, GJ
[J]. MAGNETIC RESONANCE IN MEDICINE, 1990, 14 (03) : 496 - 506
[10] EFFECTS OF HYPERCAPNIA ON BRAIN PHI AND PHOSPHATE METABOLITE REGULATION BY P-31-NMR
NISHIMURA, M
JOHNSON, DC
HITZIG, BM
OKUNIEFF, P
KAZEMI, H
[J]. JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (05) : 2181 - 2188

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