RAPID REFILLING OF CA2+ STORES IN MACROPHAGES STIMULATED BY ATP INVOLVES THE SEQUENTIAL ACTIVATION OF PHOSPHOLIPASE-D AND PROTEIN-KINASE-C

被引:9
作者
ALONSOTORRE, SR
TRAUTMANN, A
机构
[1] ECOLE NORMALE SUPER,NEUROBIOL LAB,CNRS,URA 1857,F-75005 PARIS,FRANCE
[2] UNIV VALLADOLID,FAC MED,DEPT BIOQUIM BIOL MOLEC & FISIOL,E-47005 VALLADOLID,SPAIN
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1995年 / 430卷 / 02期
关键词
CALCIUM STORES; PLATELET-ACTIVATING FACTOR; URIDINE 5'-TRIPHOSPHATE; ADENOSINE 5'-TRIPHOSPHATE; MACROPHAGES; FURA-2;
D O I
10.1007/BF00374654
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Ca2+ movements between intracellular stores, the cytoplasm and external solution were analysed in murine peritoneal macrophages stimulated by various agonists. The Ca2+ content of intracellular stores was estimated from the amplitude of Ca2+-transients elicited by ionomycin applied in Ca2+-free solution. Both uridine 5'-triphosphate (UTP) and platelet-activating factor (PAF) triggered the release of Ca2+ followed by a sustained influx, during which intracellular stores remained totally empty. In contrast, in the continuous presence of adenosine 5'-triphosphate (ATP), Ca2+ was initially released and then rapidly sequestered again by the stores. ATP-induced store refilling was not related to cell depolarization or to an increase in the intracellular Na+ concentration (two specific consequences of ATP stimulation which are not induced by PAF and UTP). Store refilling was not caused by a signal that ATP would fail to induce (e.g. as a result of receptor desensitization), but was positively controlled by ATP, even in the simultaneous presence of a concentration of PAF which, on its own, would have caused a persistent store depletion. The hypothesis that the signal delivered by ATP involves the sequential activation of phospholipase D and protein kinase C is consistent with the present pharmacological evidence. However, although we found conditions in which Ca2+ stores did not refill in the presence of ATP, this maintained store depletion was not accompanied by a sustained Ca2+ response similar to that elicited by PAF or UTP, suggesting that store depletion is a condition which is necessary, but not sufficient, for inducing Ca2+ influx.
引用
收藏
页码:230 / 237
页数:8
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