A 92-KDA HUMAN IMMUNOSTIMULATORY PROTEIN

被引：11

作者：

FONTAN, E

BRIEND, E

SAKLANIJUSFORGUES, H

DALAYER, J

VANDEKERCKHOVE, J

FAUVE, RM

机构：

[1] INST PASTEUR,MICROSEQUENCAGE PROT LAB,F-75724 PARIS 15,FRANCE

[2] STATE UNIV GHENT,PHYSIOL CHEM LAB,B-9000 GHENT,BELGIUM

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 18期

关键词：

URINE; SCID MICE; MACROPHAGES; LISTERIA MONOCYTOGENES; LEWIS CARCINOMA;

D O I：

10.1073/pnas.91.18.8353

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 mu g/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.

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页码：8353 / 8357

页数：5

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