SELECTION OF PLANT HETEROKARYONS FROM DIVERSE ORIGINS BY FLOW-CYTOMETRY

被引：17

作者：

HAMMATT, N ^{[1
]}

LISTER, A ^{[1
]}

BLACKHALL, NW ^{[1
]}

GARTLAND, J ^{[1
]}

GHOSE, TK ^{[1
]}

GILMOUR, DM ^{[1
]}

POWER, JB ^{[1
]}

DAVEY, MR ^{[1
]}

COCKING, EC ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT BOT,PLANT GENET MANIPULAT GRP,UNIV PK,NOTTINGHAM NG7 2RD,ENGLAND

来源：

PROTOPLASMA | 1990年 / 154卷 / 01期

关键词：

Electrofusion; Flow cytometry; Heterokaryon selection; Plant protoplasts; Somatic hybridization;

D O I：

10.1007/BF01349533

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplasts from a range of tissue sources for 21 species within the plant families Compositae, Cruciferae, Leguminosae, and Solanaceae were fused electrically to produce heterokaryons. Protoplasts were labelled by application of green fluorescein fluorescence to one parental protoplast population, and the red fluorescence of either naturally occurring chlorophyll or of rhodamine exogenously applied to the other parental type. Heterokaryon populations were identified, characterized and isolated using an EPICS 541 flow cytometer. Following culture, sorted heterokaryons from 11 of the 14 combinations investigated underwent division; in 5 of these combinations the heterokaryons developed into cell colonies, 4 produced callus and 2 gave rise to somatic hybrid shoots. © 1990 Springer-Verlag.

引用

页码：34 / 44

页数：11

共 45 条

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