HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MALONALDEHYDE USING P-NITROPHENYLHYDRAZINE AS A DERIVATIZING REAGENT

被引：9

作者：

KAWAI, S ^{[1
]}

FUCHIWAKI, T ^{[1
]}

HIGASHI, T ^{[1
]}

TOMITA, M ^{[1
]}

机构：

[1] KAWASAKI MED SCH, DEPT LEGAL MED, KURASHIKI, OKAYAMA 70101, JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 514卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)89373-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic procedure for the specific determination of free malonaldehyde (MA) is described. MA solution was mixed with 1 M acetate buffer (pH 3.76) and was reacted with p-nitrophenylhydrazine (NPH) hydrochloride solution in ethanol. An aliquot of the reaction mixture was injected into a C18-5 column with a mobile phase consisting of acetonitrile-isopropanol-0.01 M sodium dihydrogenphosphate (30:10:60, v/v/v) and detection at 315 nm. MA reacted readily with NPH in a weakly acidic medium at room temperature, giving 1-(p-nitrophenyl)pyrazole. The method is specific for free MA. The response was linear in the range 36-720 ng/ml of MA and the detection limit was 6 ng/ml with a 20-μl injection. © 1990.

引用

页码：29 / 35

页数：7

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