THE MULTIFUNCTIONAL FOLIC-ACID SYNTHESIS FAS GENE OF PNEUMOCYSTIS-CARINII APPEARS TO ENCODE DIHYDROPTEROATE SYNTHASE AND HYDROXYMETHYLDIHYDROPTERIN PYROPHOSPHOKINASE

被引：68

作者：

VOLPE, F

DYER, M

SCAIFE, JG

DARBY, G

STAMMERS, DK

DELVES, CJ

机构：

[1] WELLCOME RES LABS,DEPT MOLEC SCI,LANGLEY COURT,S EDEN PK RD,BECKENHAM BR3 3BS,KENT,ENGLAND

[2] UNIV EDINBURGH,INST CELL & MOLEC BIOL,DIV BIOL SCI,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

GENE | 1992年 / 112卷 / 02期

关键词：

RECOMBINANT DNA; OPPORTUNISTIC INFECTIONS; FUNGUS; METABOLIC ENZYME; NUCLEOTIDE SEQUENCE;

D O I：

10.1016/0378-1119(92)90378-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe the cloning of a multifunctional folic acid synthesis (fas) gene from Pneumocystis carinii. The nucleotide sequence contains an open reading frame interrupted by three introns, that encodes a protein of 740 amino acids with an M(r) of 97 278. The predicted Fas protein has homology to two enzyme domains, dihydropteroate synthase and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, both of which are involved in folate synthesis, and at least one other region of unknown function.

引用

页码：213 / 218

页数：6

共 16 条

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