SIGNIFICANCE OF BACTERIAL ECTOENZYMES IN AQUATIC ENVIRONMENTS

The report presents studies on temporal and spatial variations of kinetics (Vmax and Km) of bacterial ectoenzyme activity (beta-glucosidase - betaGlc, leucine aminopeptidase - Leu-amp) in the naturally eutrophic Plusssee. BetaGlc and Leu-amp activity were positively correlated with the flux of polymeric materials (polysaccharides, proteins) in the lake. BetaGlc activity was low when algal populations grew actively, but during the algal bloom breakdown betaGlc activity increased rapidly. Leu-amp displayed the highest rates of activity in the epilimnion and was tightly coupled to bacterial production. The synthesis of studied ectoenzymes was under control of a repression/derepression mechanism. The significance of ectoenzymes for the transformation and bacterial utilization of organic matter, and their role in the microbial loop in aquatic environments is discussed.