HYDROGEN-PEROXIDE GENERATION IN ORGANELLES OF EUGLENA-GRACILIS

被引：51

作者：

ISHIKAWA, T ^{[1
]}

TAKEDA, T ^{[1
]}

SHIGEOKA, S ^{[1
]}

HIRAYAMA, O ^{[1
]}

MITSUNAGA, T ^{[1
]}

机构：

[1] KINKI UNIV,DEPT FOOD & NUTR,NARA 631,JAPAN

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 06期

关键词：

EUGLENA; ALGA; H2O2; GENERATION; MITOCHONDRIA; CHLOROPLASTS;

D O I：

10.1016/0031-9422(93)85078-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen peroxide (H2O2) generation in intact mitochondria and chloroplasts was investigated in Euglena gracilis. In mitochondria, the addition of respiratory substrate caused the formation of H2O2. Lactate, the most effective substrate, yielded H2O2 at the rate of 9.5 nmol min-1 mg protein-1. Chloroplasts showed a rate of H2O2 generation of 5 mumol mgchlorophyll-1 hr-1 under illumination. The results reported here demonstrate that H2O2 is generated in mitochondria and chloroplasts and immediately diffuses from each organelle into the cytosol.

引用

页码：1297 / 1299

页数：3

共 28 条

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