SYNTHESIS OF THE CARBONIC-ACID BENZOTRIAZOL-1-YL-ESTER-(2-BIOTINYLAMINO)-9H-FLUOREN-9-YLMETHYL ESTER - A CONVENIENT TRANSIENT-BIOTINYLATION REAGENT FOR USE IN AFFINITY-CHROMATOGRAPHY

Stepwise synthesis of hydrophobic peptides frequently yields deletion products, which often require extensive purification and identification We synthesized a new transient-biotinylation reagent 9, which was conveniently used in affinify chromatography of crude products (exemplified by 11-I and 11-II), to afford pure peptides (15-1 and 15-11, respectively). Unlike conventional affinity chromatography, reagent 9 leads to free N-terminal peptides, which are thus amenable to further chemical manipulations.